ABSTRACT
This article was aimed to explore the effect ofSan-Huang (SH) decoction on improving chemosensitivity of MCF-7 cells to epirubicin and inhibition of Aurora kinase A, in order to discuss its underlying mechanism. The inhibition of MCF-7 cells proliferation on breast cancer by SH decoction was determined by CCK-8 assay. RT-PCR and western blot were used to detect the Aurora A, p53 mRNA and protein expression level of MCF-7 cells by SH decoction. The siRNA silenced Aurora A of MCF-7 cells. CCK-8 assay was used to detect the inhibition of MCF-7 cells proliferation. CCK-8 assay and AnnexinV-FITC/PI staining were used to detect the inhibition rate and apoptosis rate of MCF-7 cells treated by the combination of SH decoction and epirubicin. Western blot analysis was used to detect the expression of apoptosis-related proteins. The results showed that SH decoction inhibited the proliferation of MCF-7 cells in a dose-dependent manner (P 0.05). SH decoction can regulate the Aurora A, p53 protein and mRNA expression of MCF-7 cells. siRNA silenced Aurora A, which downregulated the inhibition rate of MCF-7 cells by SH decoction for 50.0% (from 49.2% to 24.8%). The combination of SH decoction and epirubicin enhanced the effect of epirubicin on inhibiting the proliferation rate and apoptosis rate of MCF-7 cell, regulated the expression levels of apoptosis-related protein such as c-PARP, c-Caspase 3, Bcl-2, Bax, as well as the protein level of Aurora A. It was concluded that SH decoction can increase the chemosensitivity of MCF-7 cells to epirubicin, which may be related to the inhibition of Aurora Kinase A by SH decoction.
ABSTRACT
Objective: To investigate the effects of estrogen (E 2) on the structure of madible and femur in ovariectomized rats. Methods: 18 femal SD rats were evenly divided into 3 groups:sham-ovariectomized rats (sham-OVX),ovariectonmized rats without E 2 treatment (osteoporosis,OP) and ovariectomized rats with E 2 treatment (E 2).In the group of E 2,E 2 at the dose of 10 ?g/kg was injected into abdominal cavity of the rats every the other 2 days.All rats were sacrificed after 6-week treatment.The madible and femur samples were analyzed by bone histomorphometry with QWin550CW system. Trabecular area (Tb.Ar,%), trabecular width (Tb.Wi,?m) and trabecular seperation (Tb.Sp,?m) were calculated.Result: Tb.Ar(%),Tb.Wi (?m) and Tb.Sp(?m) in the madibles in sham-OVX rats were 29.43?3.12,10.97?1.85 and 27.59?4.21;in OP 19.44?2.24,6.45?1.73 and 42.03?5.46;in E 2 treated 26.81?2.93,9.54?1.22 and 30.98?3.65,respectively.Those in the femus in sham-OVX rats were 24.19?5.53,56.72?10.36 and 132.41?47.69;in OP 12.54?4.32,40.71?8.65 and 208.67?65.39;in E 2 treated 20.08?4.86,51.38?9.07 and 159.42?56.34,respectively.Conclusion: The bone volume decreases more in femur than in madible after ovariectomy in rats.Treament of estrogen can inhibit bone absorbtion in mandible and femur.
ABSTRACT
This article mainly discusses the psychological state of senile patients in dental prosthesis on the view of medical ethics.To achieve good relationship and cooperation between doctors and patients,the author brings forward some suggestions.Doctors should make great efforts to improve their comprehensive qualificatios,show respect to patients and try to meet their reasonable demand,and moral responsibility must be strong enough when facing with senile patients.
ABSTRACT
Objective To investigate the effects on structure of madibles and femurs in ovariectomized rats. Methods Ovariectomy was performed on female SD rats, which were killed 8 weeks later. The mandibles and femurs were taken out as samples to be analyzed by bone histomorphometry and to calculate trabecular area(Tb.Ar), trabecular width(Tb.Wi), trabecular separation(Tb.Sp). Results After ovariectomy the osteopotic trabecular changes had occured in the mandibles and femurs of the rats. There was a significant difference between Tb.Ar, Tb.Wi and Tb.Sp of the mandibles and femurs in ovariectomized rats and sham-ovariectomized(P